Botulism

Clostridium botulinum growing on egg yolk agar showing the lipase reaction after 72 hours of incubation. Clostridium botulinum spores stained with Malachite Green Stain. Clostridium botulinum spores stained with Malachite Green Stain. Six week old infant with botulism. Wound botulism involvement of compound fracture of right arm. A photomicrograph of Clostridium botulinum bacteria. A photomicrograph of Clostridium botulinum type A viewed using a Gram stain technique. A photomicrograph of Clostridium botulinum type A viewed using a Gram stain technique. These were jars of contaminated Jalapeņo peppers involved in an outbreak of botulism in Pontiac, Michigan, April, 1977. This laboratorian is checking egg yolk agar for lipase-positive growth of C. botulinum colonies. This lab researcher is checking for the presence of C. botulinum during the microscopic examination of a food suspension. This laboratorian is testing for the confirmation of botulism toxin. These are Clostridium botulinum Type A colonies, Strain 2, grown on a 48hr blood agar plate. This is a Clostridium botulinum type A colony grown on a 72 hour blood agar plate; Magnification 5X. This Gram-stained micrograph of Clostridium botulinum Type-A in thioglycollate broth was incubated for 48hrs at 35°C. These are Clostridium botulinum Type E colonies displaying an opaque zone grown on a 48hr egg yolk agar plate; Mag. 1.9X. These are cultures of the anaerobic bacterium Clostridium perfringens grown on an egg yolk agar plate. This is a close up of contaminated Jalapeņo peppers involved in an outbreak of botulism in Pontiac, Michigan, April, 1977.